Hiasa H, Marians K J
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
J Biol Chem. 1999 Sep 17;274(38):27244-8. doi: 10.1074/jbc.274.38.27244.
Several protein-protein interactions have been shown to be critical for proper replication fork function in Escherichia coli. These include interactions between the polymerase and the helicase, the helicase and the primase, and the primase and the polymerase. We have studied the influence of these interactions on proper initiation at oriC by using mutant primases defective in their interaction with the helicase and DNA polymerase III holoenzyme lacking the tau subunit so that it will not interact with the helicase. We show here that accurate initiation of bidirectional DNA replication from oriC is dependent on proper placement of the primers for leading strand synthesis and is thus governed primarily by the interaction between the helicase and primase.
几种蛋白质-蛋白质相互作用已被证明对大肠杆菌中正确的复制叉功能至关重要。这些相互作用包括聚合酶与解旋酶之间、解旋酶与引发酶之间以及引发酶与聚合酶之间的相互作用。我们通过使用与解旋酶相互作用有缺陷的突变引发酶以及缺乏τ亚基从而不会与解旋酶相互作用的DNA聚合酶III全酶，研究了这些相互作用对oriC处正确起始的影响。我们在此表明，从oriC进行双向DNA复制的准确起始取决于前导链合成引物的正确定位，因此主要由解旋酶与引发酶之间的相互作用决定。
