雄性金背地松鼠冬眠期间肾上腺素刺激的脂解活性降低：一项原位微透析研究

Reduced epinephrine-stimulated lipolytic activity in male golden-mantled ground squirrel during hibernation: an in situ microdialysis study.

作者信息

Flechtner-Mors M, Ditschuneit H H, Yip I, Alt A, Torres-Schow R M, Membrano S, Caquin R, Tam C, Johnson T, Heber D

机构信息

Center for Human Nutrition, School of Medicine, University of California, Los Angeles, USA.

出版信息

Comp Biochem Physiol B Biochem Mol Biol. 1999 Jul;123(3):281-4. doi: 10.1016/s0305-0491(99)00071-1.

DOI:10.1016/s0305-0491(99)00071-1

PMID:10481256

Abstract

We studied lipolytic activities in vivo in golden mantle ground squirrels during pre-hibernation and hibernation using microdialysis technique. Microdialysis probes were inserted into the abdominal subcutaneous adipose tissues. Baseline lipolysis were assessed by measuring glycerol concentration. Epinephrine-stimulated lipolysis was also examined. Eight squirrels (four male, four female) were studied in each of the two stages. Basal glycerol concentrations were lower in the hibernating state than in the pre-hibernation state in male squirrels (P < 0.05). Epinephrine application induced glycerol release in male and female squirrels (P < 0.001) in both stages. Male squirrels demonstrated a reduced epinephrine-stimulated glycerol release in the hibernating state, which was not observed in female squirrels.

摘要

我们使用微透析技术研究了金黄腹松鼠在冬眠前和冬眠期间体内的脂解活性。将微透析探针插入腹部皮下脂肪组织。通过测量甘油浓度评估基础脂解作用。还检测了肾上腺素刺激的脂解作用。在两个阶段中，分别对8只松鼠（4只雄性，4只雌性）进行了研究。雄性松鼠在冬眠状态下的基础甘油浓度低于冬眠前状态（P < 0.05）。在两个阶段中，应用肾上腺素均能诱导雄性和雌性松鼠释放甘油（P < 0.001）。雄性松鼠在冬眠状态下肾上腺素刺激的甘油释放减少，而雌性松鼠未观察到这种情况。