Auvin-Guette C
Laboratoire de chimie des substances naturelles, ESA 8041 CNRS, 63 rue Buffon, 75005 Paris, France.
Bull Soc Pathol Exot. 2002 Aug;95(3):212-3.
Mass spectrometry is a technique for the analysis and very sensitive identification of molecules. It allows one to determine the mass of the studied product, whether pure or in a mixture, and provides some information on its molecular structure. In the particular case of peptides, this method can, under certain conditions, also provide information on the amino acid sequence. There are two complementary methods in mass spectrometry for the study of the biological molecules: i) ionisation by laser desorption assisted by matrix (MALDI) coupled to a mass analyser of the time of flight type (TOF), which is very effective for the direct study of a mixture of products and ii) ionisation by electronebulisation (ESI) coupled to mass analysers of the quadripolar type and time of flight (Qq-TOF), which allows the interfacing between high phase liquid chromatography and mass spectrometry. These two complementary techniques were already used to draw up toxin charts of snake and spider venoms. The purpose is to be able to characterise species based on an actual peptide print of poisonous gland secretions.
质谱分析法是一种用于分子分析和高灵敏度鉴定的技术。它能让人们确定所研究产物的质量，无论该产物是纯净的还是混合的，还能提供有关其分子结构的一些信息。在肽的特定情况下，这种方法在某些条件下也能提供有关氨基酸序列的信息。质谱分析法中有两种用于研究生物分子的互补方法：i）基质辅助激光解吸电离（MALDI）与飞行时间型（TOF）质量分析仪联用，这对于直接研究产物混合物非常有效；ii）电喷雾电离（ESI）与四极型和飞行时间（Qq - TOF）质量分析仪联用，这使得高效液相色谱与质谱分析法能够实现联用。这两种互补技术已被用于绘制蛇毒和蜘蛛毒液的毒素图谱。目的是能够根据毒腺分泌物的实际肽指纹图谱来鉴定物种。
