Haywood R M, Wardman P, Gault D T, Linge C
RAFT Institute of Plastic Surgery, Mount Vernon Hospital, Northwood, Middlesex, UK.
Photochem Photobiol. 1999 Sep;70(3):348-52.
Human skin biopsies (hair-bearing scalp skin and non-hair-bearing breast skin) were treated with t-butylhydroperoxide, irradiated with UV light (UVR) or irradiated with 694 nm ruby laser red light. Free-radical production and oxidative stress were assessed with electron spin resonance spectroscopy (ESR) using the ascorbate radical as a marker. In comparison with both UVR and t-butyl-hydroperoxide (which readily induce the ascorbate radical in hair-bearing and hairless skin), 694 nm red light does not result in the formation of the ascorbate radical in detectable concentrations. Spin-trapping experiments with the spin trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO) showed that while free radicals could be detected after treatment of skin with t-butylhydroperoxide, radicals could not be trapped after laser treatment. Treatment of lasered skin (containing DMPO) with t-butylhydroperoxide produced radical adducts as well as the ascorbate radical, demonstrating that the laser neither depletes endogenous ascorbate nor the preadministered spin trap. It is concluded that 694 nm red light does not induce oxidative stress in human skin in levels comparable either to t-butyl hydroperoxide or UV light.
人体皮肤活检样本（有毛发的头皮皮肤和无毛发的乳房皮肤）用叔丁基过氧化氢处理，或用紫外线（UVR）照射，或用694纳米红宝石激光红光照射。以抗坏血酸自由基为标志物，采用电子自旋共振光谱（ESR）评估自由基生成和氧化应激。与紫外线和叔丁基过氧化氢（二者均能在有毛发和无毛的皮肤中轻易诱导产生抗坏血酸自由基）相比，694纳米红光不会产生可检测浓度的抗坏血酸自由基。使用自旋捕捉剂5,5 - 二甲基 - 1 - 吡咯啉N - 氧化物（DMPO）进行的自旋捕捉实验表明，用叔丁基过氧化氢处理皮肤后可检测到自由基，但激光处理后无法捕捉到自由基。用叔丁基过氧化氢处理经激光照射的皮肤（含有DMPO）会产生自由基加合物以及抗坏血酸自由基，这表明激光既不会消耗内源性抗坏血酸，也不会消耗预先施用的自旋捕捉剂。研究得出结论，694纳米红光在人体皮肤中不会诱导产生与叔丁基过氧化氢或紫外线相当水平的氧化应激。
