Steinberg D, Mor C, Dogan H, Zacks B, Rotstein I
Department of Oral Biology, Hebrew University-Hadassah, Jerusalem, Israel.
Dent Mater. 1999 Jan;15(1):14-20. doi: 10.1016/s0109-5641(99)90026-x.
The objective of this work was to examine the effect of in vitro salivary biofilm on the adherence of oral bacteria to bleached and non-bleached restorative material (Charisma).
Charisma samples, prepared in silicon models, were treated with either 10% carbamide peroxide (CP) or 10% hydrogen peroxide (HP). After incubation with the bleaching agent for a period of one, two or three days, the samples were coated with freshly collected human saliva. The adsorption pattern of the saliva to the restorative material was determined using gel electrophoresis coupled with computerized densitometry techniques. The amount of salivary proteins adsorbed onto the treated surfaces was measured using the Bradford method. Sucrose-dependent bacterial adhesion to the salivary-coated Charisma was tested using radio-labeled Streptococcus mutans, Streptococcus sobrinus and Actinomyces viscosus. Adhesion of each bacterium to surfaces pretreated with the bleaching agents was compared with saliva coated bleached surfaces.
The profile of salivary proteins adsorption followed a similar pattern in Charisma samples pretreated with either CP or HP or untreated samples. However, the total amount of salivary proteins adsorbed onto the samples decreased after bleaching with CP or HP. Salivary biofilm, coating the surface of the restorative material, significantly decreased sucrose-dependent adhesion of Streptococcus sobrinus and Streptococcus mutans to the bleached and non-bleached surfaces, compared to non-coated specimens (p < 0.05). Saliva had a minor effect on adhesion of Actinomyces viscosus.
Our study demonstrates the importance of salivary biofilm in controlling adhesion of oral bacteria to restorative material pretreated with bleaching agents or untreated.
本研究旨在探讨体外唾液生物膜对口腔细菌黏附于漂白及未漂白修复材料（卡瑞斯玛树脂）的影响。
在硅模型中制备卡瑞斯玛树脂样本，分别用10%过氧化脲（CP）或10%过氧化氢（HP）处理。在用漂白剂孵育1、2或3天后，样本用新鲜采集的人唾液包被。使用凝胶电泳结合计算机密度测定技术确定唾液在修复材料上的吸附模式。用考马斯亮蓝法测量吸附在处理过表面的唾液蛋白量。使用放射性标记的变形链球菌、远缘链球菌和黏性放线菌测试蔗糖依赖性细菌对唾液包被的卡瑞斯玛树脂的黏附。将每种细菌对用漂白剂预处理表面的黏附与唾液包被的漂白表面进行比较。
在用CP或HP预处理的卡瑞斯玛树脂样本以及未处理样本中，唾液蛋白吸附情况遵循相似模式。然而，用CP或HP漂白后，吸附在样本上的唾液蛋白总量减少。与未包被的样本相比，覆盖在修复材料表面的唾液生物膜显著降低了远缘链球菌和变形链球菌对漂白及未漂白表面的蔗糖依赖性黏附（p < 0.05）。唾液对黏性放线菌的黏附影响较小。
我们的研究证明了唾液生物膜在控制口腔细菌黏附于经漂白剂预处理或未处理的修复材料方面的重要性。
