Lan J, Willcox M D, Jackson G D
Cooperative Research Centre for Eye Research and Technology, University of New South Wales, Sydney, New South Wales, Australia.
Aust N Z J Ophthalmol. 1999 Jun-Aug;27(3-4):218-20. doi: 10.1046/j.1440-1606.1999.00208.x.
The aims of the study were (i) to determine the immunodominant antigens of Pseudomonos aeruginoso 1 (Paer1) to tear secretory immunoglobulin A (sIgA); (ii) to study the role of sIgA in inhibiting bacterial adhesion to contact lenses.
SDS-PAGE and Western blotting were used to study the interaction of tear sIgA with Paer1; an adhesion assay was used to study the effect of sIgA on the adhesion of Paer1 to the contact lenses.
The results of our study showed that the immunodominant molecules of Paer1 which reacted with tear sIgA were of molecular masses of 105, 50, 45 kDa; the binding of sIgA to Paer1 resulted in a reduction of the adhesion of Paer1 to worn Etafilcon A contact lenses.
In conclusion, Paer1-specific sIgA are present in tears; the immunodominant molecules of Paer1 may be adhesins and tear-specific sIgA might play an important role in protecting the eye from contact-lens-induced corneal infection by preventing bacterial attachment to contact lenses and the ocular surface.
本研究的目的是（i）确定铜绿假单胞菌1型（Paer1）针对泪液分泌型免疫球蛋白A（sIgA）的免疫显性抗原；（ii）研究sIgA在抑制细菌黏附于隐形眼镜方面的作用。
采用十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）和蛋白质免疫印迹法研究泪液sIgA与Paer1的相互作用；采用黏附试验研究sIgA对Paer1黏附于隐形眼镜的影响。
我们的研究结果表明，与泪液sIgA反应的Paer1的免疫显性分子的分子量为105、50、45 kDa；sIgA与Paer1的结合导致Paer1对佩戴的依他氟康A隐形眼镜的黏附减少。
总之，泪液中存在Paer1特异性sIgA；Paer1的免疫显性分子可能是黏附素，泪液特异性sIgA可能通过防止细菌附着于隐形眼镜和眼表，在保护眼睛免受隐形眼镜引起的角膜感染方面发挥重要作用。
