Blais C, Adam A, Massicotte D, Péronnet F
Faculté de Pharmacie, Université de Montréal, Montreal H3C 3J7, H3C 3P8 Canada.
J Appl Physiol (1985). 1999 Sep;87(3):1197-201. doi: 10.1152/jappl.1999.87.3.1197.
The purpose of this study was to verify the possible appearance in the blood of bradykinin (BK) and des-Arg(9)-bradykinin (des-Arg(9)-BK) after eccentric exercise in 13 male subjects. Eccentric exercise (5 x 10 leg presses at 120% maximal voluntary concentric contraction) resulted in muscle damage and inflammation, as suggested by the significant increase in serum creatine kinase activity (from 204 +/- 41 to 322 +/- 63 U/l 12 h postexercise) and by severe lasting pain, which also peaked at 12 h postexercise. Blood BK and des-Arg(9)-BK concentrations were measured by competitive enzyme immunoassays using highly specific polyclonal rabbit IgG. Des-Arg(9)-BK concentration was not modified (preexercise: 44 +/- 14 pmol/l; pooled postexercise: 47 +/- 4 pmol/l). In contrast, BK concentration significantly increased immediately after the exercise session (68 +/- 9 vs. 42 +/- 3 pmol/l preexercise) and returned to basal values at 12, 24, and 48 h (pooled value: 40 +/- 4 pmol/l). This observation suggests that the inflammatory process due to eccentric exercise-induced muscle damage could be mediated in part by BK.
本研究的目的是验证13名男性受试者进行离心运动后，血液中缓激肽（BK）和去-精氨酸（9）-缓激肽（去-精氨酸（9）-BK）是否可能出现。离心运动（以最大自主向心收缩的120%进行5组，每组10次腿部推举）导致了肌肉损伤和炎症，血清肌酸激酶活性显著增加（运动后12小时从204±41 U/L升至322±63 U/L）以及严重的持续性疼痛（也在运动后12小时达到峰值）表明了这一点。使用高度特异性的多克隆兔IgG通过竞争性酶免疫测定法测量血液中BK和去-精氨酸（9）-BK的浓度。去-精氨酸（9）-BK的浓度没有改变（运动前：44±14 pmol/L；运动后合并值：47±4 pmol/L）。相比之下，运动后BK浓度立即显著增加（运动前为42±3 pmol/L，运动后为68±9 pmol/L），并在12、24和48小时恢复到基础值（合并值：40±4 pmol/L）。这一观察结果表明，离心运动诱导的肌肉损伤所导致的炎症过程可能部分由BK介导。
