Peters U R, Tschan M P, Kreuzer K A, Baskaynak G, Lass U, Tobler A, Fey M F, Schmidt C A
Department of Hematology/Oncology, Charité/Virchow-Clinic, Humboldt-University, Berlin, Germany.
Cancer Res. 1999 Sep 1;59(17):4233-6.
The role of the recently identified first p53-homologue, p73, in neoplastic transformation is unknown. To elucidate p73 gene expression in hematopoiesis, we investigated samples from chronic myeloid leukemia (CML) and acute myeloid leukemia patients, leukemia cell lines, as well as mature and immature normal hematopoietic cells by real-time quantitative RT-PCR and Western blot analysis. We found a distinct p73 expression profile with highest p73 mRNA transcript levels in hematopoietic malignancies such as CML blast crisis and acute myelogenous leukemia versus CML chronic phase and normal controls. Mono- and biallelic p73 expression was found in both normal and malignant hematopoiesis. p73 protein was expressed at various levels in leukemia samples and cell lines but could not be detected in any normal controls tested. Our results point to a distinct yet undefined role of p73 in the pathogenesis of myeloid neoplasms.
最近发现的首个p53同源物p73在肿瘤转化中的作用尚不清楚。为了阐明p73基因在造血过程中的表达情况，我们通过实时定量逆转录-聚合酶链反应（RT-PCR）和蛋白质免疫印迹分析，研究了慢性髓性白血病（CML）和急性髓性白血病患者的样本、白血病细胞系以及成熟和未成熟的正常造血细胞。我们发现，与CML慢性期和正常对照相比，在诸如CML急变期和急性髓性白血病等造血系统恶性肿瘤中，p73 mRNA转录水平最高，呈现出独特的p73表达谱。在正常和恶性造血过程中均发现了单等位基因和双等位基因p73表达。p73蛋白在白血病样本和细胞系中呈现不同水平的表达，但在所检测的任何正常对照中均未检测到。我们的结果表明p73在髓系肿瘤发病机制中具有独特但尚未明确的作用。
