Tschan M P, Grob T J, Peters U R, Laurenzi V D, Huegli B, Kreuzer K A, Schmidt C A, Melino G, Fey M F, Tobler A, Cajot J F
Department of Clinical Research, Institute of Medical Oncology, Bern, Switzerland.
Biochem Biophys Res Commun. 2000 Oct 14;277(1):62-5. doi: 10.1006/bbrc.2000.3627.
The p53 homologue p73 is expressed in at least six different isoforms (alpha, beta, gamma, delta, epsilon, and zeta), but unlike p53 it has rarely been found mutated in human cancers. However, altered expression of this gene has been reported in cancer cells. In order to understand if p73 is involved in normal and malignant development of myeloid cells, we investigated the expression pattern of the different p73 isoforms in progenitor and mature normal myeloid cells as well as in cells derived from acute and chronic myeloid leukemias. The results show that expression of p73 is markedly enhanced during differentiation of myeloid leukemic cells and that leukemic blasts from patients show an increased expression of the shorter p73 isoforms (gamma, delta, epsilon, zeta). In particular the epsilon isoform is only expressed in leukemic cells and completely absent in mature myeloid cells. Altogether our data suggest that p73 is involved in myeloid differentiation and its altered expression is involved in leukemic degeneration.
p53 同源物 p73 至少以六种不同的异构体（α、β、γ、δ、ε 和 ζ）形式表达，但与 p53 不同的是，它在人类癌症中很少发生突变。然而，已有报道称该基因在癌细胞中的表达发生了改变。为了了解 p73 是否参与髓系细胞的正常和恶性发育，我们研究了不同 p73 异构体在祖细胞和成熟正常髓系细胞以及急性和慢性髓系白血病来源的细胞中的表达模式。结果表明，p73 的表达在髓系白血病细胞分化过程中显著增强，并且来自患者的白血病原始细胞中较短的 p73 异构体（γ、δ、ε、ζ）表达增加。特别是 ε 异构体仅在白血病细胞中表达，在成熟髓系细胞中完全不存在。我们的数据总体表明，p73 参与髓系分化，其表达改变与白血病变性有关。
