Luque S, Mallubhotla H, Gehlert G, Kuriyel R, Dzengeleski S, Pearl S, Belfort G
Howard P. Isermann Department of Chemical Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
Biotechnol Bioeng. 1999 Nov 5;65(3):247-57.
The microfiltration performance of a novel membrane module design with helically wound hollow fibers is compared with that obtained with a standard commercial-type crossflow module containing linear hollow fibers. Cell suspensions (yeast, E. coli, and mammalian cell cultures) commonly clarified in the biotechnology industry are used for this comparison. The effect of variables such as transmembrane pressure, particle suspension concentration, and feed flow rate on membrane performance is evaluated. Normalized permeation fluxes versus flow rate or Dean number behave according to a heat transfer correlation obtained with centrifugal instabilities of the Taylor type. The microfiltration performance of this new module design, which uses secondary flows in helical tubes, is significantly better than an equivalent current commercial crossflow module when filtering suspensions relevant to the biotechnology industry. Flux and capacity improvements of up to 3.2-fold (constant transmembrane pressure operation) and 3.9-fold (constant flux operation), respectively, were obtained with the helical module over those for the linear module.
将一种具有螺旋缠绕中空纤维的新型膜组件设计的微滤性能与使用包含线性中空纤维的标准商业型错流组件所获得的微滤性能进行了比较。用于此比较的是生物技术行业中通常进行澄清处理的细胞悬浮液（酵母、大肠杆菌和哺乳动物细胞培养物）。评估了诸如跨膜压力、颗粒悬浮液浓度和进料流速等变量对膜性能的影响。归一化渗透通量与流速或Dean数的关系符合通过泰勒型离心不稳定性获得的传热关联式。这种利用螺旋管中二次流的新组件设计在过滤与生物技术行业相关的悬浮液时，其微滤性能明显优于同等的当前商业错流组件。与线性组件相比，螺旋组件在恒定跨膜压力操作下通量提高了3.2倍，在恒定通量操作下容量提高了3.9倍。
