Kluge T, Rezende C, Wood D, Belfort G
Howard P. Isermann Department of Chemical Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
Biotechnol Bioeng. 1999 Dec 20;65(6):649-58. doi: 10.1002/(sici)1097-0290(19991220)65:6<649::aid-bit5>3.0.co;2-d.
Substantially higher rates of protein and fluid volume transport for microfiltration of yeast suspensions were possible with improved hydrodynamics using centrifugal fluid instabilities called Dean vortices. Under constant permeate flux operation with suspended yeast cells, a helical module exhibited 19 times the filtration capacity of a linear module. For feed containing both BSA and beer yeast under constant transmembrane pressure with diafiltration, about twice as much protein (BSA and other proteins from cell lysis) was transported out of the feed by the helical module as compared with the linear module. The volumetric permeation flux improvements for the helical over the linear module ranged from 18 to 43% for yeast concentrations up to 4.5 dry wt %.
利用称为迪恩涡旋的离心流体不稳定性改善流体动力学后，酵母悬浮液微滤的蛋白质和流体体积传输速率显著提高。在悬浮酵母细胞的恒定渗透通量操作下，螺旋模块的过滤能力是线性模块的19倍。在恒压渗滤条件下，对于含有牛血清白蛋白（BSA）和啤酒酵母的进料，与线性模块相比，螺旋模块从进料中运出的蛋白质（BSA和细胞裂解产生的其他蛋白质）大约是其两倍。对于高达4.5干重%的酵母浓度，螺旋模块相对于线性模块的体积渗透通量提高了18%至43%。
