Cantone F A, Vandenberg J D
Plant Protection Research Unit, Plant, Soil and Nutrition Laboratory, United States Department of Agriculture/Agricultural Research Service, Tower Road, Ithaca, New York 14853, USA.
J Invertebr Pathol. 1999 Sep;74(2):193-7. doi: 10.1006/jipa.1999.4864.
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has been expressed in a variety of prokaryotic and eukaryotic organisms and has been used extensively as a marker in the study of host-pathogen interactions. We have expressed GFP in the entomopathogenic fungus Paecilomyces fumosoroseus through co-transformation with a vector that confers resistance to glufosinate ammonium. All cell types express GFP and were readily detected by fluorescence microscopy. No correlation was observed between the amount of fluorescence and the pattern of vector integration as observed by Southern analysis. Fluorescent hyphae and conidia were easily distinguished on two insect hosts, the Russian wheat aphid, Diuraphis noxia, and the diamondback moth, Plutella xylostella, and blastospores were also detected in the hemolymph of the diamondback moth. GFP-tagged strains of P. fumosoroseus can be used to study the developmental fate of the fungus within its insect hosts.
来自维多利亚多管水母的绿色荧光蛋白（GFP）已在多种原核生物和真核生物中表达，并被广泛用作宿主-病原体相互作用研究中的标记物。我们通过与赋予草铵膦抗性的载体共转化，在昆虫病原真菌玫烟色拟青霉中表达了GFP。所有细胞类型均表达GFP，通过荧光显微镜很容易检测到。通过Southern分析观察到，荧光量与载体整合模式之间没有相关性。在两种昆虫宿主，即俄罗斯小麦蚜虫麦二叉蚜和小菜蛾中，很容易区分出发荧光的菌丝和分生孢子，并且在小菜蛾的血淋巴中也检测到了芽生孢子。带有GFP标记的玫烟色拟青霉菌株可用于研究真菌在其昆虫宿主体内的发育命运。
