玫烟色拟青霉昆虫病原真菌芽生孢子的疏水和静电细胞表面特性
Hydrophobic and electrostatic cell surface properties of blastospores of the entomopathogenic fungus Paecilomyces fumosoroseus.
作者信息
Dunlap Christopher A, Biresaw Girma, Jackson Mark A
机构信息
Crop Bioprotection Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, Peoria, IL 61604, USA.
出版信息
Colloids Surf B Biointerfaces. 2005 Dec 30;46(4):261-6. doi: 10.1016/j.colsurfb.2005.11.014.
The physicochemical surface properties of blastospores of the entomopathogenic fungus Paecilomyces fumosoroseus were examined. Contact angle measurements were performed on microbial lawns composed of blastospores of P. fumosoroseus to quantify their cell surface energy components. In addition, suspensions of the blastospores were characterized with the microbial adhesion to solvents assay. Zeta potential measurements were used to quantify the surface charge and determine the zero potential of the blastospores. The results show blastospores of P. fumosoroseus are best described as having a basic monopolar surface and classified as hydrophilic. Blastospores are also negatively charged under neutral conditions with an isoelectric point of 3.4.
对昆虫病原真菌玫烟色拟青霉的芽生孢子的物理化学表面特性进行了研究。在由玫烟色拟青霉芽生孢子组成的微生物菌苔上进行接触角测量，以量化其细胞表面能成分。此外，用微生物对溶剂的黏附试验对芽生孢子悬液进行了表征。使用zeta电位测量来量化表面电荷并确定芽生孢子的零电位。结果表明，玫烟色拟青霉的芽生孢子最适合描述为具有碱性单极表面，并归类为亲水性。芽生孢子在中性条件下也带负电荷，等电点为3.4。
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