Endo T, Shikanai T, Takabayashi A, Asada K, Sato F
Division of Integrated Life Sciences, Graduate School of Biostudies, Kyoto University, Japan.
FEBS Lett. 1999 Aug 20;457(1):5-8. doi: 10.1016/s0014-5793(99)00989-8.
After a brief exposure to supra-saturating light, leaves of a tobacco transformant, in which chloroplastic NAD(P)H dehydrogenase (NDH) was defective, showed more severe photoinhibition than the wild-type, when judged by the parameter of chlorophyll fluorescence Fv/Fm. Repeated application of supra-saturating light eventually resulted in chlorosis in the NDH-defective mutant, while the wild-type sustained less photodamage and was able to recover from it. The mechanism of the phenomena is discussed with respect to the potential role of NDH in photosynthesis.
在短暂暴露于超饱和光下后,通过叶绿素荧光参数Fv/Fm判断,叶绿体NAD(P)H脱氢酶(NDH)有缺陷的烟草转化体叶片比野生型表现出更严重的光抑制。反复施加超饱和光最终导致NDH缺陷型突变体出现萎黄病,而野生型遭受的光损伤较小且能够从中恢复。针对NDH在光合作用中的潜在作用对这些现象的机制进行了讨论。