Zhou G H, Luo G A, Zhang X D
Department of Chemistry, Tsinghua University, Beijing, China.
J Chromatogr A. 1999 Aug 20;853(1-2):277-84. doi: 10.1016/s0021-9673(99)00601-9.
Microemulsion electrokinetic chromatography was used to separate a test mixture of proteins effectively. The separation was carried out in a 42.5 cm (to the detector) x 50 microns I.D. fused-silica capillary using a microemulsion system consisting of 80 mM heptane, 120 mM SDS, 900 mM butanol in 2.5 mM borate buffer, pH 8.5-9.5. Optimum separation conditions were investigated with respect to the running voltage, temperature, pH and the composition of microemulsion. Results were compared with those obtained in micellar electrokinetic chromatography and capillary zone electrophoresis. The examined method is practical and successfully applied to the assay of genetically engineering pharmaceuticals, recombinant human granulocyte macrophage colony stimulating factor injection and recombinant human granulocyte colony stimulating factor injection.
微乳液电动色谱法被用于有效分离蛋白质测试混合物。分离在一根42.5厘米（至检测器）×50微米内径的熔融石英毛细管中进行，使用的微乳液体系由80 mM庚烷、120 mM十二烷基硫酸钠、900 mM丁醇以及2.5 mM硼酸盐缓冲液（pH 8.5 - 9.5）组成。针对运行电压、温度、pH值和微乳液组成研究了最佳分离条件。将结果与在胶束电动色谱法和毛细管区带电泳中获得的结果进行了比较。所研究的方法切实可行，并成功应用于基因工程药物、重组人粒细胞巨噬细胞集落刺激因子注射液和重组人粒细胞集落刺激因子注射液的分析。
