Mrestani Y, El-Mokdad N, Rüttinger H H, Neubert R
Institute of Pharmaceutics and Biopharmaceutics, Martin-Luther-University, Halle/S., Germany.
Electrophoresis. 1998 Nov;19(16-17):2895-9. doi: 10.1002/elps.1150191616.
Electrokinetic chromatography (EKC) was introduced to determine the partitioning behavior of various cephalosporins (cefpim, cefpirom, cefaloridin, cefaclor, cephalexin, cefuroxim, cefotaxim) in microemulsions (ME) and micellar (MC) systems. The partitioning behavior of cephalosporins in microemulsions was characterized calculating the capacity factor. The required parameters for the determination of the capacity factor (micro(aq) and micro-me are the electrophoretic mobilities of the solutes in the aqueous phase and the microemulsion phase, micro(eff) is the effective mobility in the microemulsion solution) were measured by EKC using cationic and anionic microemulsion systems consisting of the surfactants/n-heptane/1-butanol/10 mM phosphate buffer solution, pH 7.0. Electrokinetic chromatography was shown to be a useful method to quantify the partitioning behavior of drugs in oil/water microemulsion. The logarithm of the capacity factor was correlated with the logarithm of the 1-octanol/water partitioning coefficients.
采用电动色谱法（EKC）测定了多种头孢菌素（头孢匹罗、头孢匹肟、头孢噻啶、头孢克洛、头孢氨苄、头孢呋辛、头孢噻肟）在微乳液（ME）和胶束（MC）体系中的分配行为。通过计算容量因子来表征头孢菌素在微乳液中的分配行为。测定容量因子所需的参数（μ(aq)和μ-me分别为溶质在水相和微乳液相中的电泳迁移率，μ(eff)为微乳液溶液中的有效迁移率）通过EKC使用由表面活性剂/正庚烷/正丁醇/10 mM pH 7.0的磷酸盐缓冲溶液组成的阳离子和阴离子微乳液体系进行测量。电动色谱法被证明是一种量化药物在油/水微乳液中分配行为的有用方法。容量因子的对数与1-辛醇/水分配系数的对数相关。
