编码犬白细胞介素-4的全长cDNA的克隆

Cloning of a full length cDNA encoding canine Interleukin-4.

作者信息

Khatlani T S, Ohno K, Ma Z, Inokuma H, Onishi T

机构信息

Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, Yoshida, Japan.

出版信息

J Vet Med Sci. 1999 Aug;61(8):967-9. doi: 10.1292/jvms.61.967.

DOI:10.1292/jvms.61.967

PMID:10487243

Abstract

Primer designed on the basis of the conserved transcription start point (tsp) region of human and bovine gene transcripts, encoding the Interleukin-4 (IL-4), and subsequently the gene specific primer and an adaptor primer pair, was successfully used, to generate the full-length complementary DNA (cDNA) sequence coding for canine IL-4 (cIL-4), from pokeweed mitogen stimulated canine peripheral blood lymphocytes. The full-length cIL-4 contains an open reading frame of 399 nucleotides (nt), with a 5' ends of 66 base pairs (bp) and 3' ends of 125 bp. The nucleotide sequence contains six possible Asn-X-Thr or Asn-X-Ser linked glycosylation sites. Five sequence motifs of TATT or ATTTA, responsible for the regulation of gene expression, are found in the 3' untranslated region.

摘要

基于人和牛基因转录本保守转录起始点（tsp）区域设计的引物，该区域编码白细胞介素-4（IL-4），随后成功使用基因特异性引物和衔接子引物对，从商陆有丝分裂原刺激的犬外周血淋巴细胞中生成编码犬IL-4（cIL-4）的全长互补DNA（cDNA）序列。全长cIL-4包含一个399个核苷酸（nt）的开放阅读框，5'端有66个碱基对（bp），3'端有125个bp。核苷酸序列包含六个可能的Asn-X-Thr或Asn-X-Ser连接的糖基化位点。在3'非翻译区发现了五个负责基因表达调控的TATT或ATTTA序列基序。