Yan Q S
Department of Biomedical and Therapeutic Sciences, University of Illinois College of Medicine at Peoria, IL 61656, USA.
Alcohol. 1999 Aug;19(1):1-7. doi: 10.1016/s0741-8329(99)00006-3.
In order to determine potential rapid changes in extracellular dopamine (DA) and 5-hydroxytryptamine (serotonin, 5-HT) during the first 5-10 min after ethanol, intracerebral microdialysis coupled with microbore HPLC was used. Ethanol at the doses of 0.5, 1, and 2 g/kg was administered IP and extracellular DA and 5-HT in 5-min dialysates from the nucleus accumbens (NAC) of freely moving rats were determined. Ethanol at all doses significantly increased extracellular DA and 5-HT. DA and 5-HT peaked at 10, 15, 20 min and 20, 20, 55 min following 0.5, 1, and 2 g/kg, respectively. Based on previous reports showing that brain or blood ethanol levels peaked within 10-20 min after administration, the results indicate that peak increases in extracellular DA and 5-HT in the NAC occurred at approximately the same time, with the DA time course more temporally correlated with the blood or brain alcohol concentration curve reported in the literature. These results support the concept that the reinforcing properties of ethanol derive, at least in part, from its ability to stimulate DA release in the NAC. In addition, the present data are not in opposition with a role of 5-HT in the ethanol reinforcing effect.
为了确定乙醇注射后最初5 - 10分钟内细胞外多巴胺（DA）和5 - 羟色胺（血清素，5 - HT）的潜在快速变化，采用了脑内微透析结合微径高效液相色谱法。以0.5、1和2 g/kg的剂量腹腔注射乙醇，并测定自由活动大鼠伏隔核（NAC）中5分钟透析液中的细胞外DA和5 - HT。所有剂量的乙醇均显著增加细胞外DA和5 - HT。0.5、1和2 g/kg乙醇注射后，DA分别在10、15、20分钟达到峰值，5 - HT分别在20、20、55分钟达到峰值。基于先前的报道显示脑或血乙醇水平在给药后10 - 20分钟内达到峰值，结果表明NAC中细胞外DA和5 - HT的峰值增加大约同时出现，DA的时间进程与文献中报道的血或脑酒精浓度曲线在时间上更相关。这些结果支持了乙醇的强化特性至少部分源于其刺激NAC中DA释放的能力这一概念。此外，目前的数据并不反对5 - HT在乙醇强化作用中的作用。
