Yoshimoto K, McBride W J, Lumeng L, Li T K
Department of Psychiatry, Indiana University School of Medicine, Indianapolis.
Alcohol Clin Exp Res. 1992 Aug;16(4):781-5. doi: 10.1111/j.1530-0277.1992.tb00678.x.
The effects of intraperitoneal administration of ethanol, 0.5, 1.0, and 2.0 g/kg body weight, on the extracellular concentrations of dopamine (DA), serotonin (5-HT), and their major metabolites were studied in the nucleus accumbens (ACC) of alcohol-naive, selectively bred high-alcohol-drinking (HAD) and low-alcohol-drinking (LAD) lines of rats using the technique of microdialysis. In both lines, the extracellular levels of DA were increased following the injection of 1.0 and 2.0 g of ethanol/kg body weight while only the 2.0-g/kg dose elevated the concentration of 5-HT. None of the ethanol doses altered the extracellular levels of the major metabolites of DA and 5-HT. Dose-response curves for DA and 5-HT release indicated no marked difference in the sensitivity to ethanol between the lines. Local perfusion with 60 mM K+ through the microdialysis probe markedly enhanced the release of DA and 5-HT in the ACC of both lines; there was a small difference between the lines in the amounts of DA, but not 5-HT, released by K(+)-stimulation. Overall, the results indicate that (1) the ACC DA system is more sensitive than the ACC 5-HT system to intraperitoneal ethanol administration in both lines and (2) there is no evidence for a relationship between alcohol preference and sensitivity of the ACC DA and 5-HT systems to acute intraperitoneal ethanol administration.
采用微透析技术，研究了腹腔注射0.5、1.0和2.0 g/kg体重乙醇对初饮酒精的、选择性培育的高饮酒量（HAD）和低饮酒量（LAD）品系大鼠伏隔核（ACC）中多巴胺（DA）、5-羟色胺（5-HT）及其主要代谢产物细胞外浓度的影响。在两个品系中，注射1.0和2.0 g乙醇/kg体重后，DA的细胞外水平均升高，而只有2.0 g/kg剂量升高了5-HT的浓度。乙醇各剂量均未改变DA和5-HT主要代谢产物的细胞外水平。DA和5-HT释放的剂量反应曲线表明，两个品系对乙醇的敏感性无明显差异。通过微透析探针用60 mM K+进行局部灌注，显著增强了两个品系ACC中DA和5-HT的释放；K+刺激释放的DA量在两个品系之间存在微小差异，但5-HT无差异。总体而言，结果表明：（1）在两个品系中，ACC DA系统比ACC 5-HT系统对腹腔注射乙醇更敏感；（2）没有证据表明饮酒偏好与ACC DA和5-HT系统对急性腹腔注射乙醇的敏感性之间存在关联。
