禽腺病毒FAV-1（CELO）基因组的早期转录

[Early transcription of FAV-1 (CELO) avian adenovirus genome].

作者信息

Tikhomirov E E, Baburina T M, Neugodova G L, Doronin K K, Zakharchuk A N

出版信息

Vopr Virusol. 1998 Nov-Dec;43(6):279-83.

Abstract

The patterns of RNA from cells infected with avian adenovirus chicken embryo lethal orphan (CELO) virus were analyzed by Northern hybridization method. Early RNAs are specifically hybridized with several fragments of the Eco-RI restriction map: A (49-81%), B (0.2-20.3%), D (81-92.5%), and E fragments of the Bam-HI CELO DNA restriction map. Early RNAs were not hybridized with C (37.8-49%), G (31-37.8%), and E (20.3-31%) fragments of the Eco-RI DNA CELO restriction map. At least 19 types of virus-specific RNA molecules homologous to 4 different regions of the virus genome are produced in CELO-infected permissive cells. Several classes of virus-specific RNAs were identified in CELO virus-transformed cell strains.

摘要

采用Northern杂交法分析了感染禽腺病毒鸡胚致死孤儿（CELO）病毒的细胞中的RNA模式。早期RNA与Eco - RI限制图谱的几个片段特异性杂交：Bam - HI CELO DNA限制图谱的A（49 - 81%）、B（0.2 - 20.3%）、D（81 - 92.5%）和E片段。早期RNA未与Eco - RI DNA CELO限制图谱的C（37.8 - 49%）、G（31 - 37.8%）和E（20.3 - 31%）片段杂交。在CELO感染的允许细胞中产生了至少19种与病毒基因组4个不同区域同源的病毒特异性RNA分子。在CELO病毒转化的细胞株中鉴定出了几类病毒特异性RNA。