Chiocca S, Kurzbauer R, Schaffner G, Baker A, Mautner V, Cotten M
Institute for Molecular Pathology, Vienna, Austria.
J Virol. 1996 May;70(5):2939-49. doi: 10.1128/JVI.70.5.2939-2949.1996.
The complete DNA sequence of the avian adenovirus chicken embryo lethal orphan (CELO) virus (FAV-1) is reported here. The genome was found to be 43,804 bp in length, approximately 8 kb longer than those of the human subgenus C adenoviruses (Ad2 and Ad5). This length is supported by pulsed-field gel electrophoresis analysis of genomes isolated from several related FAV-1 isolates (Indiana C and OTE). The genes for major viral structural proteins (Illa, penton base, hexon, pVI, and pVIII), as well as the 52,000-molecular-weight (52K) and 100K proteins and the early-region 2 genes and IVa2, are present in the expected locations in the genome. CELO virus encodes two fiber proteins and a different set of the DNA-packaging core proteins, which may be important in condensing the longer CELO virus genome. No pV or pIX genes are present. Most surprisingly, CELO virus possesses no identifiable E1, E3, and E4 regions. There is 5 kb at the left end of the CELO virus genome and 15 kb at the right end with no homology to Ad2. The sequences are rich in open reading frames, and it is likely that these encode functions that replace the missing El, E3, and E4 functions.
本文报道了禽腺病毒鸡胚致死孤儿（CELO）病毒（FAV-1）的完整DNA序列。发现该基因组长度为43,804 bp，比人类C亚属腺病毒（Ad2和Ad5）的基因组约长8 kb。从几种相关的FAV-1分离株（印第安纳C株和OTE株）中分离的基因组进行脉冲场凝胶电泳分析，证实了这一长度。主要病毒结构蛋白（IIIa、五邻体基底、六邻体、pVI和pVIII）以及52,000分子量（52K）和100K蛋白、早期区域2基因和IVa2基因，在基因组中的预期位置上均有存在。CELO病毒编码两种纤维蛋白和一组不同的DNA包装核心蛋白，这可能对浓缩更长的CELO病毒基因组很重要。不存在pV或pIX基因。最令人惊讶的是，CELO病毒没有可识别的E1、E3和E4区域。CELO病毒基因组左端有5 kb，右端有15 kb与Ad2没有同源性。这些序列富含开放阅读框，很可能这些序列编码的功能替代了缺失的E1、E3和E4功能。
