Kubrusly M S, Matheucci Júnior E, Leite K R, Coelho A M, Monte O, Machado M C, Pinotti H W
Gastroenterology Surgery Department, Faculty of Medicine São Paulo University.
Rev Hosp Clin Fac Med Sao Paulo. 1999 Jan-Feb;54(1):17-20. doi: 10.1590/s0041-87811999000100004.
Mutations at codons 12, 13, or 61 of the H-ras, K-ras, and N-ras have been detected in human neoplasias by a variety of techniques. Some of these techniques are very sensitive and can detect K-ras mutation in 90% of the cases of pancreatic adenocarcinomas. We analyzed 11 samples of pancreatic adenocarcinoma, three samples of pancreatic mucinous cystadenoma, and two samples without tumors in formalin-fixed paraffin embedded tissue sections. K-ras mutations at codon 12 were detected by a two-step PCR-enriched technique in all the samples of pancreatic adenocarcinoma, but not in cystadenoma or control samples. This technique may be useful for early detection of pancreatic cancer.
通过多种技术已在人类肿瘤中检测到H-ras、K-ras和N-ras基因第12、13或61密码子的突变。其中一些技术非常灵敏，能在90%的胰腺腺癌病例中检测到K-ras突变。我们分析了11份福尔马林固定石蜡包埋组织切片中的胰腺腺癌样本、3份胰腺黏液性囊腺瘤样本以及2份无肿瘤样本。采用两步PCR富集技术在所有胰腺腺癌样本中检测到了第12密码子的K-ras突变，但在囊腺瘤或对照样本中未检测到。该技术可能有助于胰腺癌的早期检测。
