用于特异性检测火鸡出血性肠炎病毒（一种禽腺病毒）的聚合酶链反应。
PCR for specific detection of haemorrhagic enteritis virus of turkeys, an avian adenovirus.
作者信息
Hess M, Raue R, Hafez H M
机构信息
Institut für Geflügelkrankheiten, Freie Universität Berlin, Germany.
出版信息
J Virol Methods. 1999 Aug;81(1-2):199-203. doi: 10.1016/s0166-0934(99)00067-1.
A hexon gene based PCR was developed for specific amplification of DNA sequences from the haemorrhagic enteritis virus (HEV) of turkeys. The hexon genes of different avian adenoviruses were compared for primer construction. Two regions with low sequence homology between HEV and fowl adenovirus (FAV) hexon genes were selected for primer localisation. In correlation with the known sequence data a fragment of 1647 bp was amplified from a live vaccine and spleens of turkeys suffering from haemorrhagic enteritis (HE). All other avian adenoviruses which are able to infect turkeys, i.e. FAV and turkey adenoviruses (TAV), were negative. This is the first PCR for specific detection of HEV DNA which should be useful for rapid diagnosis and epidemiological investigations of HEV infections in turkeys.
开发了一种基于六邻体基因的聚合酶链反应（PCR），用于特异性扩增来自火鸡出血性肠炎病毒（HEV）的DNA序列。比较了不同禽腺病毒的六邻体基因以构建引物。在HEV和禽腺病毒（FAV）六邻体基因之间选择了两个序列同源性低的区域进行引物定位。根据已知序列数据，从一种活疫苗和患有出血性肠炎（HE）的火鸡脾脏中扩增出一个1647 bp的片段。所有其他能够感染火鸡的禽腺病毒，即FAV和火鸡腺病毒（TAV），均为阴性。这是首次用于特异性检测HEV DNA的PCR，对火鸡HEV感染的快速诊断和流行病学调查应具有重要意义。
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