Nazerian K, Fadly A M
Avian Dis. 1987 Apr-Jun;31(2):234-40.
Isolation of hemorrhagic enteritis virus (HEV) from spleens of infected turkeys in the MDTC-RP19 lymphoblastoid cell line was compared with detection of HEV antigen in the same spleens using the agar gel precipitation (AGP) test. A concordance of 80% was found between the two assays. Virus isolation had a sensitivity of 84% and a specificity of 88% compared with the AGP test. RP19 cells were also susceptible to infection with several other avian adenoviruses, but such infection was easily differentiated from that of HEV by a fluorescent-antibody (FA) test. Turkeys required 10(2) tissue-culture-infectious doses (TCID) to develop HE-specific lesions and 10(5) TCID to be killed. On the other hand, as little as 10 TCID of apathogenic HEV protected the poults against challenge with virulent HEV. The enzyme-linked immunosorbent assay (ELISA) for detection of HEV antibody was improved by using virus-infected RP19 cells as antigen. The ELISA appears to be more sensitive than the serum-neutralization test.
将从感染火鸡脾脏中分离出血性肠炎病毒（HEV）并接种于MDTC-RP19淋巴母细胞系，与使用琼脂凝胶沉淀（AGP）试验检测同一脾脏中的HEV抗原进行了比较。两种检测方法的一致性为80%。与AGP试验相比，病毒分离的敏感性为84%，特异性为88%。RP19细胞也易被其他几种禽腺病毒感染，但通过荧光抗体（FA）试验可轻松将这种感染与HEV感染区分开来。火鸡需要10²组织培养感染剂量（TCID）才能出现HE特异性病变，需要10⁵TCID才能致死。另一方面，低至10 TCID的无致病性HEV就能保护雏火鸡免受强毒HEV的攻击。通过使用病毒感染的RP19细胞作为抗原，改进了用于检测HEV抗体的酶联免疫吸附测定（ELISA）。ELISA似乎比血清中和试验更敏感。
