藤黄微球菌B-P 26的十一异戊二烯基二磷酸合酶的结晶及初步X射线衍射研究
Crystallization and preliminary X-ray diffraction studies of undecaprenyl diphosphate synthase from Micrococcus luteus B-P 26.
作者信息
Fujihashi M, Shimizu N, Zhang Y W, Koyama T, Miki K
机构信息
Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
出版信息
Acta Crystallogr D Biol Crystallogr. 1999 Sep;55(Pt 9):1606-7. doi: 10.1107/s0907444999008768.
Undecaprenyl diphosphate synthase from Micrococcus luteus B-P 26, one of the Z-prenyl chain-elongating enzymes, was crystallized using the sitting-drop vapour-diffusion method with ammonium sulfate and lithium sulfate as precipitants. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 127.2, b = 60.2, c = 75.7 A, beta = 105.6 degrees. The crystals diffract X-rays to at least 2.2 A resolution using synchrotron radiation and are suitable for high-resolution crystal structure analysis.
藤黄微球菌B-P 26中的十一异戊二烯基二磷酸合酶是一种Z-异戊二烯链延长酶，采用坐滴气相扩散法，以硫酸铵和硫酸锂作为沉淀剂进行结晶。晶体属于单斜空间群C2，晶胞参数为a = 127.2、b = 60.2、c = 75.7 Å，β = 105.6°。这些晶体利用同步辐射X射线衍射分辨率至少达到2.2 Å，适合进行高分辨率晶体结构分析。
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