Pérez J, Day M J, Martín M P, González S, Mozos E
Departamento de Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria de Córdoba, Spain.
Vet Immunol Immunopathol. 1999 Jul 1;69(1):33-46. doi: 10.1016/s0165-2427(99)00032-x.
The distribution of T lymphocytes (CD3+), B lymphocytes (CD79+), immunoglobulin-containing plasma cells (IgG, IgM and IgA), macrophages (Mac387+) and MHC Class II antigen was analysed in the inflammatory infiltrate associated with cutaneous squamous cell carcinomas (SCC) from 23 cats. Peri-tumoural skin (12 cases) and precancerous lesions of actinic keratosis (nine cases) were also evaluated for the expression of MHC Class II. The results revealed that an abundant inflammatory infiltrate was associated with the majority of SCC. This infiltrate was composed mainly of CD3+ T lymphocytes, B cells (CD79+) and IgG-bearing plasma cells, and the intensity of infiltration increased with the degree of invasiveness of the tumour. The number of CD3+ T cells and CD79+ cells was significantly increased in well-differentiated SCC compared with moderately differentiated tumours, whereas the number of IgM+, IgA+ plasma cells and Mac387+ macrophages was low or moderate and did not change significantly with histologic grade or invasiveness. MHC Class II antigen was expressed by infiltrating lymphocytes and macrophages, and by fibroblasts. A variable number of neoplastic cells (10% to 80%) in 10 SCC, and keratinocytes of basal layers in seven of nine cases of actinic keratosis also expressed MHC Class II, whereas keratinocytes of normal skin were always negative for this antigen. These results suggest that CD3+ T lymphocytes, CD79+ B cells and IgG-bearing plasma cells may participate in down-regulation of tumour growth, since these cell types were particularly numerous in well-differentiated and mildly invasive SCC, as well as in actinic keratosis. The expression of MHC Class II by neoplastic cells could enhance this local anti-tumour immune response.
分析了23只猫皮肤鳞状细胞癌（SCC）相关炎性浸润中T淋巴细胞（CD3+）、B淋巴细胞（CD79+）、含免疫球蛋白的浆细胞（IgG、IgM和IgA）、巨噬细胞（Mac387+）和MHC II类抗原的分布情况。还评估了12例肿瘤周围皮肤和9例光化性角化病癌前病变中MHC II类的表达情况。结果显示，大多数SCC伴有丰富的炎性浸润。这种浸润主要由CD3+ T淋巴细胞、B细胞（CD79+）和含IgG的浆细胞组成，浸润强度随肿瘤侵袭程度增加而增强。与中度分化肿瘤相比，高分化SCC中CD3+ T细胞和CD79+细胞数量显著增加，而IgM+、IgA+浆细胞和Mac387+巨噬细胞数量低或中等，且不随组织学分级或侵袭性显著变化。MHC II类抗原由浸润的淋巴细胞、巨噬细胞和成纤维细胞表达。10例SCC中有数量不等的肿瘤细胞（10%至80%），9例光化性角化病中有7例的基底层角质形成细胞也表达MHC II类，而正常皮肤的角质形成细胞该抗原始终为阴性。这些结果表明，CD3+ T淋巴细胞、CD79+ B细胞和含IgG的浆细胞可能参与肿瘤生长的下调，因为这些细胞类型在高分化和轻度侵袭性SCC以及光化性角化病中特别多。肿瘤细胞表达MHC II类可增强这种局部抗肿瘤免疫反应。
