[抗体结构规则：人单克隆IgA1免疫球蛋白（骨髓瘤蛋白Tro）的一级结构，II. 用溴化氰裂解单体IgA分子以及还原和烷基化的重链和轻链（作者译）]
[Rule of antibody structure: the primary structure of a human monoclonal IgA1-immunoglobulin (myeloma protein Tro), II. Cleavage of the monomer IgA-molecule and the reduced and alkylated H- and L-chains by cyanogen bromide (author's transl)].
作者信息
Ruban E, Starościk K, Janusz M, Kratzin H, Hilschmann N
出版信息
Hoppe Seylers Z Physiol Chem. 1978 Dec;359(12):1689-95.
PMID:104913
Abstract
The monomer of myeloma protein Tro as well as the reduced and alkylated H- and L-chains were cleaved by cyanogen bromide. All cyanogen-bromide fragments were isolated and characterized by amino acid analyses, end-group and molecular weight determinations. The 4 smaller fragments of the 5 H-chain fragments were split with trypsin. The peptides were isolated and their primary structure was determined.
摘要
骨髓瘤蛋白Tro的单体以及还原和烷基化的重链和轻链均被溴化氰裂解。所有溴化氰片段均经分离，并通过氨基酸分析、端基分析和分子量测定进行表征。重链的5个片段中的4个较小片段用胰蛋白酶裂解。分离出这些肽并确定了它们的一级结构。
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[Rule of antibody structure: the primary structure of a human monoclonal IgA1-immunoglobulin (myeloma protein Tro), II. Cleavage of the monomer IgA-molecule and the reduced and alkylated H- and L-chains by cyanogen bromide (author's transl)].[抗体结构规则：人单克隆IgA1免疫球蛋白（骨髓瘤蛋白Tro）的一级结构，II. 用溴化氰裂解单体IgA分子以及还原和烷基化的重链和轻链（作者译）]
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