Salotra P, Raina A, Ramesh V
Molecular Biology Laboratory, Institute of Pathology (ICMR), Safdarjung Hospital, New Delhi, India.
Trans R Soc Trop Med Hyg. 1999 Jan-Feb;93(1):98-101. doi: 10.1016/s0035-9203(99)90197-9.
Sera from 32 Indian patients with post-kala-azar dermal leishmaniasis (PKDL) were examined for antibodies by immunoblot analysis using an antigen extract of Leishmania donovani. The study revealed that the humoral immune response in PKDL patients was quite distinct compared to that in kala-azar patients. Antibodies to 3 antigens of L. donovani (molecular sizes 110, 65 and 38-42 kDa) were predominant in a majority (78%) of PKDL patients. The most important finding was the consistent recognition of 2 parasite antigens (of 110 and 65 kDa) by PKDL sera; antibodies to the 110-kDa antigen were detectable in 97% of cases, while antibodies to the 65-kDa antigen were detectable in 100% of cases that were examined. None of the 18 cases of leprosy, 10 of vitiligo, or the 30 healthy persons included in the study showed antibodies to these 2 antigens. Thus Western blot analysis provided a highly sensitive test for PKDL patients. Further, it led to the identification of 2 parasite antigens (110 and 65 kDa) that elicit an antibody response in 97-100% of PKDL patients. Purified or recombinant versions of these proteins deserve consideration as potential target antigens in development of simpler, highly specific and sensitive serodiagnostic tests for PKDL.
采用杜氏利什曼原虫抗原提取物，通过免疫印迹分析检测了32例印度黑热病后皮肤利什曼病（PKDL）患者血清中的抗体。研究表明，与黑热病患者相比，PKDL患者的体液免疫反应有很大差异。多数（78%）PKDL患者中，针对杜氏利什曼原虫3种抗原（分子大小分别为110、65和38 - 42 kDa）的抗体占主导。最重要的发现是PKDL血清一致识别2种寄生虫抗原（110 kDa和65 kDa）；97%的病例中可检测到针对110 kDa抗原的抗体，而在所有检测的病例中，100%可检测到针对65 kDa抗原的抗体。该研究纳入的18例麻风病患者、10例白癜风患者及30名健康人中，均未显示出针对这2种抗原的抗体。因此，免疫印迹分析为PKDL患者提供了一种高度敏感的检测方法。此外，该分析还鉴定出2种寄生虫抗原（110 kDa和65 kDa），97% - 100%的PKDL患者会对其产生抗体反应。这些蛋白质的纯化或重组形式，有望作为开发更简单、高特异性和高敏感性PKDL血清诊断检测方法的潜在靶抗原。
