Tamura K, Yonei-Tamura S, Izpisúa Belmonte J C
The Salk Institute for Biological Studies, Gene expression Laboratory, 10010 N. Torrey Pines Road, La Jolla, CA 92037-1099, USA.
Mech Dev. 1999 Sep;87(1-2):181-4. doi: 10.1016/s0925-4773(99)00126-4.
In here we report the identification of two new members of the T-box gene family, zf-tbx5 and zf-tbx4, from the Zebrafish, Danio rerio. The amino acid sequences within the T-box domain share high homology with the mouse, chick, and newt orthologs. Whole mount in situ hybridization revealed specific expression of these genes in the eye and Fin buds. zf-tbx5 expression is restricted to the pectoral Fin bud, whilst zf-tbx4 transcripts are confined in the pelvic Fin bud. These results reveal the conserved expression pattern of Tbx5 and Tbx4 during appendage development in all animal species studied to date.
在此我们报告从斑马鱼（Danio rerio）中鉴定出T-box基因家族的两个新成员，即zf-tbx5和zf-tbx4。T-box结构域内的氨基酸序列与小鼠、鸡和蝾螈的直系同源物具有高度同源性。整体原位杂交显示这些基因在眼睛和鳍芽中特异性表达。zf-tbx5的表达局限于胸鳍芽，而zf-tbx4的转录本则局限于腹鳍芽。这些结果揭示了迄今为止在所有研究的动物物种中，Tbx5和Tbx4在附肢发育过程中保守的表达模式。
