Johnstone I B
Department of Biomedical Sciences, University of Guelph, Ontario.
Can Vet J. 1999 Sep;40(9):645-8.
A new in vitro von Willebrand factor-collagen binding activity (vWF:CBA) assay was used to assess qualitative changes in vWF in normal dogs and dogs with Type I von Willebrand's disease (vWD) following treatment with desmopressin acetate (DDAVP). Although DDAVP induced increases in vWF antigen concentrations at 1 hour postinfusion in both normal and vWD dogs (57% and 60% increases, respectively), there were disproportionately greater increases in vWF:CBA (96% and 103% increases). These results support the hypothesis that the enhanced hemostatic activity induced by DDAVP is, at least in part, due to the selective release of more functionally active vWF multimers. The assay, as described, provides a convenient means of simultaneously assessing vWF quantity and function before and after DDAVP administration.
一种新的体外血管性血友病因子 - 胶原结合活性（vWF:CBA）检测方法被用于评估正常犬和患有I型血管性血友病（vWD）的犬在接受醋酸去氨加压素（DDAVP）治疗后vWF的定性变化。尽管在输注后1小时，DDAVP使正常犬和vWD犬的vWF抗原浓度均升高（分别升高57%和60%），但vWF:CBA的升高幅度却不成比例地更大（分别升高96%和103%）。这些结果支持了这样一种假说，即DDAVP诱导的增强止血活性至少部分是由于选择性释放了更多功能活性的vWF多聚体。所述检测方法提供了一种在给予DDAVP之前和之后同时评估vWF数量和功能的便捷手段。
