Johnstone I B
Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Canada.
J Vet Diagn Invest. 1999 Jul;11(4):308-13. doi: 10.1177/104063879901100402.
A sensitive enzyme-linked immunosorbent assay was used for the simultaneous assessment of the amount of von Willebrand factor (vWF) in canine plasma and its ability to bind to canine collagen in vitro. In 60 normal dogs, there was close correlation between the concentration of vWF and its activity as determined by vWF-collagen binding. In 14 dogs with type I expressions of von Willebrand's disease, the ratio of vWF antigen to collagen binding activity was normal or only slightly increased. In 7 dogs with type II expressions of the disease, this ratio was consistently elevated suggesting a significant functional deficiency of the protein. Plasma from 3 dogs with type III von Willebrand's disease had little collagen binding activity because of the severe quantitative deficiency of the protein. The described assay permits the rapid assessment of both the quantity and quality of vWF in a dog. This information is necessary for the detection and characterization of canine von Willebrand's disease, particularly the type II expressions, which cannot be diagnosed by quantitative vWF assays alone.
采用一种灵敏的酶联免疫吸附测定法，同时评估犬血浆中血管性血友病因子（vWF）的含量及其体外与犬胶原蛋白结合的能力。在60只正常犬中，vWF浓度与其通过vWF-胶原蛋白结合测定的活性之间存在密切相关性。在14只患有I型血管性血友病的犬中，vWF抗原与胶原蛋白结合活性的比值正常或仅略有升高。在7只患有II型该病的犬中，该比值持续升高，提示该蛋白存在明显的功能缺陷。3只患有III型血管性血友病的犬的血浆由于该蛋白严重的定量缺乏而几乎没有胶原蛋白结合活性。所描述的测定法可快速评估犬体内vWF的数量和质量。这些信息对于犬血管性血友病的检测和特征鉴定是必要的，尤其是II型表现，仅通过定量vWF测定无法诊断。
