一种与人类gadd45和小鼠MyD118高度同源的新型人类cDNA——GRP17的分子克隆、表达及定位

Molecular cloning, expression, and mapping of a novel human cDNA, GRP17, highly homologous to human gadd45 and murine MyD118.

作者信息

Suzuki M, Watanabe T K, Fujiwara T, Takahashi E, Tanigami A

机构信息

Otsuka GEN Research Institute, Otsuka Pharmaceutical Co. Ltd., Tokushima, Japan.

出版信息

J Hum Genet. 1999;44(5):300-3. doi: 10.1007/s100380050164.

DOI:10.1007/s100380050164

PMID:10496071

Abstract

From a human placenta cDNA library, we isolated a novel gene whose predicted product is highly homologous in amino acid sequence to human gadd45 and murine MyD118 proteins (about 55% and 52% identity, respectively). The cDNA clone, designated GRP17 (gadd-related protein, 17 kDa), contained a 477-bp open reading frame encoding 159 amino acids. Northern blot analysis revealed strong expression of a 1.35-kb transcript in heart, placenta, liver, skeletal muscle, prostate, testis, and ovary. A 1.7-kb additional transcript was detected in liver. We assigned the GRP17 gene to chromosome 9q22.1-q22.2 by fluorescence in situ hybridization (FISH).

摘要

从人胎盘cDNA文库中，我们分离出一个新基因，其预测产物的氨基酸序列与人类gadd45和小鼠MyD118蛋白高度同源（分别约有55%和52%的同一性）。该cDNA克隆被命名为GRP17（gadd相关蛋白，17 kDa），包含一个477 bp的开放阅读框，编码159个氨基酸。Northern印迹分析显示，在心脏、胎盘、肝脏、骨骼肌、前列腺、睾丸和卵巢中，有一个1.35 kb的转录本强烈表达。在肝脏中检测到一个额外的1.7 kb转录本。我们通过荧光原位杂交（FISH）将GRP17基因定位到9号染色体的q22.1 - q22.2区域。