感染华支睾吸虫的豚鼠和大鼠的虫体回收率及沉淀素抗体反应

Worm recovery and precipitin antibody response in guinea pigs and rats infected with Clonorchis sinensis.

作者信息

Su K E, Wang F Y, Chi P Y

机构信息

Department of Parasitology, College of Medicine, National Taiwan University, Taipei, ROC.

出版信息

J Microbiol Immunol Infect. 1998 Dec;31(4):211-6.

PMID:10496161

Abstract

Guinea pigs (Hartley strain) and rats (Wistar strain) were each fed 200 and 100 Clonorchis sinensis metacercariae, respectively. Five animals from each species were sacrificed weekly between 1-8 weeks postinfection (WPI) and then at 12, 16, 20 and 30 WPI for collection of worms, bile and sera. The overall worm recovery rates for guinea pigs and rats were 18.7% and 12.4%, respectively. Only one of the five rats examined at 20 WPI still harbored one worm, while all were worm-free at 30 WPI. By a double diffusion test, no antibodies were detected against C. sinensis adult antigens in the bile juice. Serum antibodies were detected in at least 95% of the infected guinea pigs between 4-30 WPI and rats between 3-16 WPI. Precipitin antibodies seemed to be correlated with the presence of live worms in rats that had been infected for more than 12 weeks.

摘要

豚鼠（哈特利品系）和大鼠（Wistar品系）分别感染200条和100条华支睾吸虫囊蚴。在感染后1至8周（WPI）期间，每周从每个物种中处死5只动物，然后在12、16、20和30 WPI时处死，以收集虫体、胆汁和血清。豚鼠和大鼠的总体虫体回收率分别为18.7%和12.4%。在20 WPI检查的5只大鼠中，只有1只仍有1条虫体，而在30 WPI时所有大鼠均无虫体。通过双向扩散试验，在胆汁中未检测到针对华支睾吸虫成虫抗原的抗体。在4至30 WPI的至少95%感染豚鼠和3至16 WPI的大鼠中检测到血清抗体。沉淀素抗体似乎与感染超过12周的大鼠体内活虫的存在相关。