Dienes-Nagy A, Rivier L, Giroud C, Augsburger M, Mangin P
Institut Universitaire de Médecine Légale, Lausanne, Switzerland.
J Chromatogr A. 1999 Aug 27;854(1-2):109-18. doi: 10.1016/s0021-9673(99)00750-5.
Simultaneous determination of opiates and their glucuronides in body fluids has a great practical interest in the forensic assessment of heroin intoxication. A selective and sensitive method for quantification of morphine and its 3- and 6-glucuronides, codeine, codeine glucuronide and 6-monoacetylmorphine (6-MAM) based on liquid chromatography-electrospray ionisation mass spectrometry is described. The drugs were analysed in human autopsy whole blood after solid-phase extraction on a C8 cartridge. The separation was performed on an ODS column in acetonitrile (analysis time 15 min). For the quantitative analysis, deuterated analogues of each compound were used as internal standards. Selected-ion monitoring was applied where the molecular ion was chosen for quantification. The limits of quantification were 0.5 ng/ml for morphine and 6-MAM and 1 ng/ml for the 6-glucuronide of morphine, codeine-6-glucuronide and codeine and 5 ng/ml for the 3-glucuronide of morphine.
在海洛因中毒的法医鉴定中，同时测定体液中的阿片类药物及其葡萄糖醛酸苷具有重大实际意义。本文描述了一种基于液相色谱 - 电喷雾电离质谱法的选择性和灵敏方法，用于定量测定吗啡及其3 - 和6 - 葡萄糖醛酸苷、可待因、可待因葡萄糖醛酸苷和6 - 单乙酰吗啡（6 - MAM）。在C8柱上进行固相萃取后，对人体尸检全血中的药物进行分析。分离在ODS柱上以乙腈进行（分析时间15分钟）。对于定量分析，每种化合物的氘代类似物用作内标。采用选择离子监测，选择分子离子进行定量。吗啡和6 - MAM的定量限为0.5 ng/ml，吗啡、可待因 - 6 - 葡萄糖醛酸苷和可待因的6 - 葡萄糖醛酸苷的定量限为1 ng/ml，吗啡的3 - 葡萄糖醛酸苷的定量限为5 ng/ml。
