Kakuno T, Hiura H, Yamashita J, Bartsch R G, Horio T
J Biochem. 1978 Dec;84(6):1649-51. doi: 10.1093/oxfordjournals.jbchem.a132294.
Hydrogenase was easily solubilized from light-grown cells of R. rubrum with 10 mM Na ethylenediaminetetraacetate. The enzyme thus obtained was so stable that loss of its activity was undetectable during storage at room temperature for 6 months under air atmosphere, provided that NaCl, KCl or CsCl was present at greater than or equal to 0.7 M.