Hirua H, Kakuno T, Yamashita J, Bartsch R G, Horio T
J Biochem. 1979 Oct;86(4):1151-3. doi: 10.1093/oxfordjournals.jbchem.a132610.
With Rhodospirillum rubrum, hydrogenase was found to exist partly as an extracellular enzyme in the culture medium. After 4-day cultivation, the total activity and the specific activity of the enzyme in the medium were about 10 times and 230 times as high as those in the crude extract obtained from disrupted cells. The time course for the production of hydrogenase during cultivation was studied.
对于红螺菌,发现氢化酶部分以细胞外酶的形式存在于培养基中。培养4天后,培养基中该酶的总活性和比活性分别约为从破碎细胞获得的粗提物中该酶总活性和比活性的10倍和230倍。研究了培养过程中氢化酶产生的时间进程。