Vasicová P, Pátek M, Nesvera J, Sahm H, Eikmanns B
Institute of Microbiology, Academy of Sciences of the Czech Republic, CZ-14220 Praha 4, Czech Republic.
J Bacteriol. 1999 Oct;181(19):6188-91. doi: 10.1128/JB.181.19.6188-6191.1999.
Deletion and mutational analysis of the promoter P-dapA from Corynebacterium glutamicum was performed to identify regions and particular nucleotides important for its function. An extended -10 region and a stretch of six T's at positions -55 to -50 were found to be the most important elements in the promoter function. The results of mutational analysis of P-dapA are consistent with the conclusions of statistical computer-aided analysis of 44 C. glutamicum promoter sequences.