枯草芽孢杆菌σA依赖型启动子序列的汇编与分析：RNA聚合酶与上游启动子DNA之间存在广泛接触的证据
Compilation and analysis of Bacillus subtilis sigma A-dependent promoter sequences: evidence for extended contact between RNA polymerase and upstream promoter DNA.
作者信息
Helmann J D
机构信息
Section of Microbiology, Cornell University, Ithaca, NY 14853-8101, USA.
出版信息
Nucleic Acids Res. 1995 Jul 11;23(13):2351-60. doi: 10.1093/nar/23.13.2351.
Abstract
Sequence analysis of 236 promoters recognized by the Bacillus subtilis sigma A-RNA polymerase reveals an extended promoter structure. The most highly conserved bases include the -35 and -10 hexanucleotide core elements and a TG dinucleotide at position -15, -14. In addition, several weakly conserved A and T residues are present upstream of the -35 region. Analysis of dinucleotide composition reveals A2- and T2-rich sequences in the upstream promoter region (-36 to -70) which are phased with the DNA helix: An tracts are common near -43, -54 and -65; Tn tracts predominate at the intervening positions. When compared with larger regions of the genome, upstream promoter regions have an excess of An and Tn sequences for n > 4. These data indicate that an RNA polymerase binding site affects DNA sequence as far upstream as -70. This sequence conservation is discussed in light of recent evidence that the alpha subunits of the polymerase core bind DNA and that the promoter may wrap around RNA polymerase.
摘要
对枯草芽孢杆菌σA-RNA聚合酶识别的236个启动子进行序列分析，揭示了一种扩展的启动子结构。最保守的碱基包括-35和-10六核苷酸核心元件以及-15、-14位置的TG二核苷酸。此外，在-35区域上游存在几个弱保守的A和T残基。对二核苷酸组成的分析揭示了上游启动子区域（-36至-70）中富含A2和T2的序列，这些序列与DNA螺旋呈相位关系：在-43、-54和-65附近常见An序列；Tn序列在中间位置占主导。与基因组的更大区域相比，对于n>4，上游启动子区域有过量的An和Tn序列。这些数据表明，RNA聚合酶结合位点对上游至-70的DNA序列有影响。根据最近的证据讨论了这种序列保守性，该证据表明聚合酶核心的α亚基结合DNA，并且启动子可能围绕RNA聚合酶。
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