猪链球菌7型快速聚合酶链反应检测
Rapid PCR test for Streptococcus suis serotype 7.
作者信息
Smith H E, van Bruijnsvoort L, Buijs H, Wisselink H J, Smits M A
机构信息
Department of Bacteriology, DLO-Institute for Animal Science and Health, AB Lelystad, The Netherlands.
出版信息
FEMS Microbiol Lett. 1999 Sep 15;178(2):265-70. doi: 10.1111/j.1574-6968.1999.tb08686.x.
Recent epidemiological studies on Streptococcus suis infections in pigs indicated that, besides serotypes 1, 2 and 9, serotype 7 is also frequently associated with diseased animals. For the latter serotype, however, no rapid and sensitive diagnostic methods are available. This hampers prevention and control programs. Here, we describe the development of a type-specific PCR test for the rapid and sensitive detection of S. suis serotype 7. The test is based on DNA sequences of capsular (cps) genes specific for serotype 7. These sequences were identified by cross-hybridization of several individual cps genes with the chromosomal DNAs of 35 different S. suis serotypes.
近期针对猪感染猪链球菌的流行病学研究表明，除了1型、2型和9型血清型外，7型血清型也常与患病动物相关。然而，对于后一种血清型，尚无快速且灵敏的诊断方法。这阻碍了预防和控制计划。在此，我们描述了一种针对猪链球菌7型血清型的快速灵敏检测的型特异性聚合酶链反应（PCR）检测方法的开发。该检测基于7型血清型特异性的荚膜（cps）基因的DNA序列。这些序列是通过几个单独的cps基因与35种不同猪链球菌血清型的染色体DNA进行交叉杂交而鉴定出来的。
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