Dinelli G, Lucchese C
Department of Agronomy, University of Bologna, Italy.
Electrophoresis. 1999 Sep;20(12):2524-32. doi: 10.1002/(SICI)1522-2683(19990801)20:12<2524::AID-ELPS2524>3.0.CO;2-W.
In recent years variety discrimination has been achieved in a range of agricultural crops by means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Previous works on genus Lolium have shown the effectiveness of this technique for the topic of cultivar identification. In the present research the potential of capillary gel electrophoresis (CGE) for identification of Italian (Lolium multiflorum Lam.), annual (Lolium rigidum Gaud.) and perennial (Lolium perenne L.) ryegrass cultivars in comparison with SDS-PAGE was investigated. Separation conditions of SDS-CGE were chosen in order to obtain electrophoretic data comparable with those of SDS-PAGE, at the expense of analysis speed. Both peak area and migration time of SDS-CGE electropherograms were reproducible. In the examined cultivars, a total of 27 (16.8-96.8 kDa) and 28 (13.2-111.5 kDa) protein subunits were detected by SDS-PAGE and SDS-CGE, respectively. The variability in seed storage protein composition was processed by numerical taxonomy. All cultivars were clearly identified by both electrophoresis systems. The orthogonality between SDS-PAGE and SDS-CGE was suggested by the lack of correlation among protein profiles obtained with the two separation systems. The nonredundant information from these analytical systems should provide a relevant benefit for identification of Lolium cultivars and wild biotypes that are extremely uniform phenotypically.
近年来，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）已在一系列农作物中实现了品种鉴别。先前关于黑麦草属的研究表明了该技术在品种鉴定方面的有效性。在本研究中，研究了毛细管凝胶电泳（CGE）与SDS-PAGE相比在鉴定意大利黑麦草（多花黑麦草）、一年生黑麦草（硬直黑麦草）和多年生黑麦草品种方面的潜力。选择SDS-CGE的分离条件是为了获得与SDS-PAGE可比的电泳数据，但以分析速度为代价。SDS-CGE电泳图谱的峰面积和迁移时间都是可重复的。在所检测的品种中，SDS-PAGE和SDS-CGE分别检测到27个（16.8 - 96.8 kDa）和28个（13.2 - 111.5 kDa）蛋白质亚基。通过数值分类法处理种子贮藏蛋白组成的变异性。两种电泳系统都能清楚地鉴定所有品种。两种分离系统获得的蛋白质图谱之间缺乏相关性，这表明SDS-PAGE和SDS-CGE之间具有正交性。这些分析系统的非冗余信息应为鉴定表型极其一致的黑麦草品种和野生生物型提供相关益处。
