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通过十二烷基硫酸钠凝胶电泳和毛细管电泳区分羽扇豆属物种的基因型。

Distinction between genotypes of Lupinus species by sodium dodecyl sulphate-gel electrophoresis and by capillary electrophoresis.

作者信息

Pollard N J, Wrigley C W, Bekes F, Aumatell A, MacRitchie F

机构信息

CSIRO Grain Quality Research Laboratory, Division of Plant Industry, North Ryde, Australia.

出版信息

Electrophoresis. 1996 Jan;17(1):221-3. doi: 10.1002/elps.1150170137.

DOI:10.1002/elps.1150170137
PMID:8907544
Abstract

Effective distinction was achieved among a wide range of lupin grain samples by either sodium dodecyl sulphate (SDS)-gel electrophoresis or capillary electrophoresis, based on grain-protein composition. Capillary electrophoresis was faster (< 1 h) and provided slightly greater distinction between the samples. On the other hand, SDS-gel electrophoresis could provide a greater through-put of samples in a 24 h period. Either technique could be used successfully to distinguish between lupin species and cultivars for taxonomic analysis or seed identification.

摘要

基于谷物蛋白质组成,通过十二烷基硫酸钠(SDS)-凝胶电泳或毛细管电泳,在多种羽扇豆谷物样品之间实现了有效区分。毛细管电泳速度更快(<1小时),并且在样品之间提供了稍大的区分度。另一方面,SDS-凝胶电泳在24小时内可以处理更多的样品。这两种技术都可以成功用于区分羽扇豆的种类和品种,以进行分类分析或种子鉴定。

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