Pollard N J, Wrigley C W, Bekes F, Aumatell A, MacRitchie F
CSIRO Grain Quality Research Laboratory, Division of Plant Industry, North Ryde, Australia.
Electrophoresis. 1996 Jan;17(1):221-3. doi: 10.1002/elps.1150170137.
Effective distinction was achieved among a wide range of lupin grain samples by either sodium dodecyl sulphate (SDS)-gel electrophoresis or capillary electrophoresis, based on grain-protein composition. Capillary electrophoresis was faster (< 1 h) and provided slightly greater distinction between the samples. On the other hand, SDS-gel electrophoresis could provide a greater through-put of samples in a 24 h period. Either technique could be used successfully to distinguish between lupin species and cultivars for taxonomic analysis or seed identification.
基于谷物蛋白质组成,通过十二烷基硫酸钠(SDS)-凝胶电泳或毛细管电泳,在多种羽扇豆谷物样品之间实现了有效区分。毛细管电泳速度更快(<1小时),并且在样品之间提供了稍大的区分度。另一方面,SDS-凝胶电泳在24小时内可以处理更多的样品。这两种技术都可以成功用于区分羽扇豆的种类和品种,以进行分类分析或种子鉴定。