Rodríguez Esteban C, Capdevila J, Economides A N, Pascual J, Ortiz A, Izpisúa Belmonte J C
The Salk Institute for Biological Studies, Gene Expression Laboratory, La Jolla, California 92037, USA.
Nature. 1999 Sep 16;401(6750):243-51. doi: 10.1038/45738.
In the chick embryo, left-right asymmetric patterns of gene expression in the lateral plate mesoderm are initiated by signals located in and around Hensen's node. Here we show that Caronte (Car), a secreted protein encoded by a member of the Cerberus/Dan gene family, mediates the Sonic hedgehog (Shh)-dependent induction of left-specific genes in the lateral plate mesoderm. Car is induced by Shh and repressed by fibroblast growth factor-8 (FGF-8). Car activates the expression of Nodal by antagonizing a repressive activity of bone morphogenic proteins (BMPs). Our results define a complex network of antagonistic molecular interactions between Activin, FGF-8, Lefty-1, Nodal, BMPs and Car that cooperate to control left-right asymmetry in the chick embryo.
在鸡胚中，侧板中胚层基因表达的左右不对称模式是由位于亨森结及其周围的信号启动的。我们在此表明，Caronte（Car）是一种由刻耳柏洛斯/丹（Cerberus/Dan）基因家族成员编码的分泌蛋白，它介导了侧板中胚层中依赖音猬因子（Shh）的左侧特异性基因的诱导。Car由Shh诱导，并被成纤维细胞生长因子8（FGF - 8）抑制。Car通过拮抗骨形态发生蛋白（BMPs）的抑制活性来激活Nodal的表达。我们的结果定义了一个由激活素、FGF - 8、Lefty - 1、Nodal、BMPs和Car之间相互拮抗的分子相互作用组成的复杂网络，这些相互作用共同控制鸡胚中的左右不对称性。
