Poglitsch C L, Meredith G D, Gnatt A L, Jensen G J, Chang W H, Fu J, Kornberg R D
Department of Structural Biology, Stanford University School of Medicine, California 94305, USA.
Cell. 1999 Sep 17;98(6):791-8. doi: 10.1016/s0092-8674(00)81513-5.
The structure of an actively transcribing complex, containing yeast RNA polymerase II with associated template DNA and product RNA, was determined by electron crystallography. Nucleic acid, in all likelihood the "transcription bubble" at the active center of the enzyme, occupies a previously noted 25 A channel in the protein structure. Details are indicative of a roughly 90 degrees bend of the DNA between upstream and downstream regions. The DNA apparently lies entirely on one face of the polymerase, rather than passing through a hole to the opposite side, as previously suggested.
通过电子晶体学确定了一个正在进行转录的复合物的结构，该复合物包含酵母RNA聚合酶II以及相关的模板DNA和产物RNA。核酸很可能是酶活性中心的“转录泡”，占据了蛋白质结构中一个先前已被注意到的25埃通道。细节表明DNA在上游和下游区域之间有大约90度的弯曲。DNA显然完全位于聚合酶的一个面上，而不是像先前推测的那样穿过一个孔到达另一侧。
