Ozmen J, Bobryshev Y V, Lord R S
Surgical Professorial Unit, St. Vincent's Hospital, University of, New South Wales, Australia.
Cardiovasc Surg. 2001 Aug;9(4):329-33. doi: 10.1016/s0967-2109(00)00141-1.
We have previously identified dendritic cells in the intima of human large arteries and stenotic vein coronary bypass grafts. The mechanisms by which these dendritic cells might regulate immune responses in atherosclerotic lesions and stenotic vein grafts are unknown. The aim of the present study was to investigate whether dendritic cells in carotid plaques and in stenotic aortocoronary vein grafts express an immunoregulatory molecule CD40. Segments of wall from eight carotid arteries and three stenotic aortocoronary saphenous vein grafts were obtained at operation. CD40+ cells were detected in all specimens of both occluded and stenotic grafts and carotid plaques. Although CD40+ cells of various cell types were intermingled in most areas within the plaques and stenotic grafts, there were sites where CD40+ cells were located in small groups. Consecutive sections demonstrated that a small population of CD40+ cells stained positively for S100. These CD40+/S100+ cells were clustered within the intima but were also found in the media and adventitia. This suggests that dendritic cells, which accumulate within vessels affected by atherosclerosis and graft disease, express CD40 co-stimulatory molecule. The expression of CD40 molecule on the dendritic cells may be important in regulating T cell responses within atherosclerotic plaques and stenotic vein grafts.
我们之前已在人类大动脉内膜和狭窄的静脉冠状动脉旁路移植血管中鉴定出树突状细胞。这些树突状细胞调节动脉粥样硬化病变和狭窄静脉移植物中免疫反应的机制尚不清楚。本研究的目的是调查颈动脉斑块和狭窄的主动脉冠状动脉静脉移植物中的树突状细胞是否表达免疫调节分子CD40。手术中获取了8条颈动脉壁段和3条狭窄的主动脉冠状动脉大隐静脉移植物。在所有闭塞和狭窄移植物以及颈动脉斑块标本中均检测到CD40+细胞。尽管在斑块和狭窄移植物内的大多数区域，各种细胞类型的CD40+细胞相互混杂，但也有一些部位CD40+细胞呈小群分布。连续切片显示，一小部分CD40+细胞S100染色呈阳性。这些CD40+/S100+细胞聚集在内膜内，但也见于中膜和外膜。这表明，在受动脉粥样硬化和移植物疾病影响的血管内积聚的树突状细胞表达CD40共刺激分子。树突状细胞上CD40分子的表达可能在调节动脉粥样硬化斑块和狭窄静脉移植物内的T细胞反应中起重要作用。
