Badr W H, Loghmanee D, Karalus R J, Murphy T F, Thanavala Y
Department of Molecular Immunology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
Vaccine. 1999 Aug 20;18(1-2):29-37. doi: 10.1016/s0264-410x(99)00166-8.
The kinetics of the anti-P6 antibody response was characterized in three strains of mice of different haplotypes (Balb/c; H-2d, C3H/H; H-2k, SJL/J; H-2s). Anti-P6 antibodies were measured on a weekly basis by enzyme-linked immunosorbent assay (ELISA). The primary response peaked 2 or 3 weeks after the initial injection with 40 microg of purified P6. The response remained at a plateau for 8-10 weeks. A maximum titer of 1:1,638,400 was attained and then steadily declined. To study the ability of P6 to generate a recall response, we opted to boost the vaccinated mice with a known subimmunogenic dose of live nontypeable Haemophilus influenzae (NTHI) bacteria. After the anti-P6 antibody titers in the primed animals had stayed at baseline levels for 2 weeks, the mice were injected intraperitonealy with 10(8) cfu of NTHI in sterile saline. This challenge with live NTHI bacteria induced a very rapid and strong secondary antibody response in all mice. Finally, we demonstrated that these murine anti-P6 sera were 100% bactericidal against three strains of NTHI when tested in a complement dependant bactericidal assay.
在三种不同单倍型的小鼠品系（Balb/c；H-2d、C3H/H；H-2k、SJL/J；H-2s）中对抗P6抗体反应的动力学进行了表征。通过酶联免疫吸附测定（ELISA）每周检测一次抗P6抗体。初次反应在初次注射40微克纯化P6后2或3周达到峰值。反应在8至10周内保持平稳。达到了1:1,638,400的最大滴度，然后稳步下降。为了研究P6产生回忆反应的能力，我们选择用已知的亚免疫原性剂量的非分型流感嗜血杆菌（NTHI）活菌对已接种疫苗的小鼠进行加强免疫。在致敏动物中的抗P6抗体滴度在基线水平保持2周后，将小鼠腹腔注射10(8) cfu的NTHI于无菌盐水中。这种用活的NTHI细菌进行的激发在所有小鼠中诱导了非常快速和强烈的二次抗体反应。最后，我们证明，当在补体依赖性杀菌试验中进行测试时，这些鼠抗P6血清对三种NTHI菌株具有100%的杀菌作用。
