Nelsen-Salz Birgit, Eggers Hans J, Zimmermann Holger
Institut für Virologie der Universität zu Köln, Fürst-Pückler-Str. 56, 50935 Köln, Germany1.
J Gen Virol. 1999 Sep;80 ( Pt 9):2311-2313. doi: 10.1099/0022-1317-80-9-2311.
The enterovirus echovirus 9 strain Barty (E9/Barty) is pathogenic for newborn mice as well as for humans. In contrast to the apathogenic prototype strain Hill, strain Barty encodes an RGD motif in the C-terminal part of the structural protein VP1. Data are presented that show that E9/Barty binds its target cells via contact of the RGD motif to the alpha(v)beta3 integrin (vitronectin receptor), whereas prototype Hill uses a different, still unidentified receptor site. Furthermore, virus titres of murine muscle tissue were compared after infection of newborn and 1-, 2-, 3- and 12-week-old mice. The replication capacity of the virus decreased dramatically with age of the infected mice. Since E9/Barty does not replicate or replicates only poorly in mice older than about 5 days, and expression of the vitronectin receptor is reported to be down-regulated in striated muscle tissue during development, it is suggested that susceptibility of mice to this echovirus infection is controlled by the availability of alpha(v)beta3 integrin.
肠道病毒埃可病毒9型巴蒂株（E9/Barty）对新生小鼠和人类均具有致病性。与无致病性的原型株希尔不同，巴蒂株在结构蛋白VP1的C末端编码一个RGD基序。现有数据表明，E9/Barty通过RGD基序与α(v)β3整合素（玻连蛋白受体）接触来结合其靶细胞，而原型株希尔使用的是一个不同的、尚未确定的受体位点。此外，在感染新生小鼠以及1周龄、2周龄、3周龄和12周龄小鼠后，比较了小鼠肌肉组织中的病毒滴度。病毒的复制能力随感染小鼠年龄的增长而显著下降。由于E9/Barty在约5日龄以上的小鼠中不复制或仅能低效复制，并且据报道玻连蛋白受体的表达在发育过程中会在横纹肌组织中下调，因此有人提出小鼠对这种埃可病毒感染的易感性受α(v)β3整合素可用性的控制。
