Zhao D, Wang Y, Zhao J
Institute of Botany, Chinese Academy of Sciences, Beijing, China.
Chin J Biotechnol. 1998;14(3):141-7.
The effects of some physical and chemical factors on callus growth and flavonoids formation in callus cultures of Saussurea medusa Maxim were studied. The optimal temperature for callus growth and flavonoids formation were 25 degrees C. Flavonoids formation were greatly inhibited under dark condition, and stimulated by white light. Callus growth was promoted when 1 mg/L NAA and 0.2 mg/L KT were added to the media. The combination of 5% sucrose and 1% glucose was useful for callus growth and flavonoids formation. A fine callus strain of S.medusa was selected through irradiation with 4000 Gy of gamma-ray. The growth rate and flavonoids content of the callus strain were high and stable. Sensitive and rapid methods of high-performance liquid chromatography as well as UV spectrophotometry were developed for detecting the flavonoids in cultured cells. The results revealed that the content of total flavonoids in dry cells under white light condition was 3.2%, 4.4 times more than under dark condition and the control of flavonoids in dry cell culture at 25 degrees C was 2.02% which was 5 and 3.2 times more than those cultured at 20 degrees C and 35 degrees C respectively.
研究了一些理化因素对水母雪兔子愈伤组织培养中愈伤组织生长及黄酮类化合物形成的影响。愈伤组织生长和黄酮类化合物形成的最适温度为25℃。黄酮类化合物的形成在黑暗条件下受到极大抑制，而在白光下受到促进。当向培养基中添加1mg/L萘乙酸（NAA）和0.2mg/L激动素（KT）时，愈伤组织生长得到促进。5%蔗糖和1%葡萄糖的组合有利于愈伤组织生长和黄酮类化合物的形成。通过4000戈瑞的γ射线辐照筛选出了一株优良的水母雪兔子愈伤组织菌株。该愈伤组织菌株的生长速率和黄酮类化合物含量高且稳定。建立了灵敏、快速的高效液相色谱法和紫外分光光度法用于检测培养细胞中的黄酮类化合物。结果表明，白光条件下干细胞中总黄酮含量为3.2%，比黑暗条件下高4.4倍，25℃下干细胞培养物中黄酮类化合物的对照含量为2.02%，分别比20℃和35℃下培养的高5倍和3.2倍。
