Li J, Wang T, Yang X, Zhang J
Colloge of Life Science, Henan Normal University, Xinxiang 453002.
Zhong Yao Cai. 2000 May;23(5):249-51.
Callus were induced from the leaves and stems of Hypericum perforatum L. The cell suspension culture and plate culture were also investigated in this paper. The results showed that the medium of MS which contained 2,4-D 1 mg/L and NAA 1 mg/L was the best one for inducing callus. The plant growth regulator of 2,4-D was assential for callus and suspension culture. Factors such as the time of cell suspension culture, the ways of plating and cell density, which affects the plating efficency were studied. The results showed that plating at density of 5 x 10(3) cells/ml and with monocells seperated from the cells cultured 14-21 days, high plating efficiency could be obtained either by conditional plate culture or by nursing plate culture.
从贯叶连翘的叶片和茎诱导出愈伤组织。本文还研究了细胞悬浮培养和平板培养。结果表明，含有1mg/L 2,4-D和1mg/L NAA的MS培养基是诱导愈伤组织的最佳培养基。2,4-D这种植物生长调节剂对愈伤组织和悬浮培养至关重要。研究了影响平板培养效率的细胞悬浮培养时间、接种方式和细胞密度等因素。结果表明，细胞接种密度为5×10³个/ml，且单细胞分离自培养14 - 21天的细胞，无论是采用条件平板培养还是看护平板培养，均可获得较高的平板培养效率。
