Seki M, Iida A, Morikawa H
Laboratory of Plant Molecular Biology, Instutuite of Physical and Chemical Research (RIKEN), Tsukuba Life Science Center, Ibraki, Japan.
Mol Biotechnol. 1999 Jun;11(3):251-5. doi: 10.1007/BF02788683.
Particle bombardment has proved to be useful for the transformation of plants. We have previously reported successful transient expression of the beta-glucuronidase (GUS) gene in cultured plant cells and tissues and the stable transformation of various plants using a pneumatic particle gun. In this chapter, we describe transient expression of the GUS gene in Arabidopsis thaliana leaves and roots using the pneumatic particle gun.
事实证明，粒子轰击法对于植物转化很有用。我们之前曾报道过，使用气动粒子枪在培养的植物细胞和组织中成功实现了β-葡萄糖醛酸酶（GUS）基因的瞬时表达，并实现了多种植物的稳定转化。在本章中，我们描述了使用气动粒子枪在拟南芥叶片和根中实现GUS基因的瞬时表达。
