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经粒子轰击后，超过 90%表达β-葡萄糖醛酸酶的细胞直接将外源基因导入其细胞核的证据。

Evidence That More than 90% of beta-Glucuronidase-Expressing Cells after Particle Bombardment Directly Receive the Foreign Gene in their Nucleus.

机构信息

Department of Biological Sciences, Faculty of Science, Hiroshima University, Hiroshima 730, Japan.

出版信息

Plant Physiol. 1991 Oct;97(2):829-31. doi: 10.1104/pp.97.2.829.

DOI:10.1104/pp.97.2.829
PMID:16668476
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1081084/
Abstract

Plasmid DNA harboring the beta-glucuronidase (GUS) gene, coated on gold particles, was delivered into cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells using a pneumatic particle gun. Cytological analyses of intracellular location of the introduced gold particles before and after GUS expression assay indicated that more than 90% of GUS-expressing cells after bombardment received a DNA-coated particle in their nucleus.

摘要

携带β-葡萄糖醛酸酶（GUS）基因的质粒 DNA 包裹在金颗粒上，使用气动粒子枪递送到培养的烟草（Nicotiana tabacum L. cv Bright Yellow-2）细胞中。在 GUS 表达分析之前和之后对细胞内引入的金颗粒的位置进行细胞分析表明，轰击后超过 90%的 GUS 表达细胞在其核内接收了包裹 DNA 的颗粒。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae92/1081084/65768ef352a4/plntphys00697-0364-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae92/1081084/65768ef352a4/plntphys00697-0364-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae92/1081084/65768ef352a4/plntphys00697-0364-a.jpg

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Cell. 1980 Nov;22(2 Pt 2):479-88. doi: 10.1016/0092-8674(80)90358-x.
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利用粒子轰击法在花生（Arachis hypogaea L.）种子外植体中瞬时表达来自巴西坚果的GUS和2S白蛋白基因。
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